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laser puller sutter instrument p-2000  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company laser puller sutter instrument p-2000
    Laser Puller Sutter Instrument P 2000, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laser+puller+sutter+instrument+p-2000/micropipette+puller+p+97/pm40641299-60-75-77
    Average 90 stars, based on 1 article reviews
    laser puller sutter instrument p-2000 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Temperature Regulation:

    Article Title: Coaxial tips for a scanning microwave microscope and its calibration with dielectric references
    Article Snippet: .. Next, the capillary is placed in a laser puller (Sutter Instrument P-2000), and a good bond between the glass and metal is formed by applying heat while simultaneously evacuating the capillary via attached silicon tubes. ..

    High Performance Liquid Chromatography:

    Article Title: A proximity-dependent biotinylation map of a human cell.
    Article Snippet: Christopher D. Go, James D. R. Knight, Archita Rajasekharan, Bhavisha Rathod, Geoffrey G. Hesketh, Kento T. Abe, Ji-Young Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y. Zhu, Evelyn Popiel, Jean-Philippe Lambert, Étienne Coyaud, Sally W. T. Cheung, Dushyandi Rajendran, Cassandra J. Wong, Hana Antonicka, Laurence Pelletier, Alexander F. Palazzo, Eric A. Shoubridge, Brian Raught & Anne-Claude Gingras1,2 ✉

    Generated:

    Article Title: Involvement of the AKT Pathway in Resistance to Erlotinib and Cabozantinib in Triple-Negative Breast Cancer Cell Lines.
    Article Snippet: .. Coated nano-spray emitters were generated from fused silica capillary tubing, with 75 μm internal diameter, 365 μm outer diameter, and 5–8 μm tip opening, using a laser puller (Sutter Instrument Co., Novato, CA, USA, model P-2000, with parameters set as heat: 280, FIL = 0, VEL = 18, DEL = 2000). .. Nano-spray emitters were packed with C18 reversed-phase material (Reprosil-Pur 120 C18-AQ, 1.9 μm) and resuspended in methanol using a pressure injection cell.

    Article Title: Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes
    Article Snippet: The column was then pre-equilibrated in buffer A (6 μl) before being connected in-line to a NanoLC-Ultra 2D plus HPLC system (Eksigent) coupled to a LTQ-Orbitrap Velos (Thermo Electron) equipped with a nanoelectrospray ion source (Proxeon Biosystems). .. Nano-spray emitters were generated from fused silica capillary tubing, with 75μm internal diameter, 365μm outer diameter and 5-8μm tip opening, using a laser puller (Sutter Instrument Co., model P-2000, with parameters set as heat: 280, FIL = 0, VEL = 18, DEL = 2000). .. Nano-spray emitters were packed with C18 reversed-phase material (Reprosil-Pur 120 C18-AQ, 3μm) resuspended in methanol using a pressure injection cell.

    Article Title: Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila .
    Article Snippet: .. Coated nano-spray emitters were generated from fused silica capillary tubing (75 μm ID, 365 μm OD) with a 5–8 μm tip opening, using a laser puller (Sutter Instrument Co., model P-2000). .. Nano-spray emitters were packed with C18 reversedphase material (Reprosil-Pur 120 C18-AQ, 3 μm) and resuspended in methanol using a pressure injection cell.

    Article Title: Optimizing Proximity Proteomics on the EvoSep-timsTOF LC-MS System.
    Article Snippet: All samples were acquired via data-dependent acquisition (DDA) LC–MS/MS using an Eksigent ekspert nanoLC 425 coupled to a TripleTOF 6600 Instrument (AB SCIEX, Concord, ON, Canada). .. Samples were resuspended in 5% formic acid, and aliquots equivalent to 1/8 and 1/4 of each sample (which were derived from one 150 mm plate, resulting in initial pellet masses of ∼50 mg) were directly loaded on a 100 μm × 15-cm nanospray emitter with a 5–8 μm tip opening at 800 nL/min for 20 min. Nanospray emitters were generated from fused silica capillary tubing (100 μm inner diameter, 365 μm outer diameter) using a laser puller (Sutter Instrument Co., model P-2000) and were packed with C18 reversed-phase material (Reprosil-Pur 120 C18AQ, 3 μm) in methanol using a pressure injection cell. .. Each pellet contained approximately 1 mg of biotinylated protein, as estimated using a peptide concentration assay of a streptavidin pulldown of the miniTurbo-3×FLAG-EGFP sample using a NanoDrop OneC (Thermo Fisher Scientific, Waltham, MA, USA) against a standard curve of a bovine serum albumin digest.

    Transferring:

    Article Title: Probing Non-Faradaic Process during Elastic Deformation in a Single Sphere of Extremely Soft Mesoporous Carbon
    Article Snippet: .. A pipette was prepared by pulling a glass capillary with a laser puller (Sutter Instrument, P-2000) and adjusted to an opening of 2 μm. ..

    Derivative Assay:

    Article Title: Optimizing Proximity Proteomics on the EvoSep-timsTOF LC-MS System.
    Article Snippet: All samples were acquired via data-dependent acquisition (DDA) LC–MS/MS using an Eksigent ekspert nanoLC 425 coupled to a TripleTOF 6600 Instrument (AB SCIEX, Concord, ON, Canada). .. Samples were resuspended in 5% formic acid, and aliquots equivalent to 1/8 and 1/4 of each sample (which were derived from one 150 mm plate, resulting in initial pellet masses of ∼50 mg) were directly loaded on a 100 μm × 15-cm nanospray emitter with a 5–8 μm tip opening at 800 nL/min for 20 min. Nanospray emitters were generated from fused silica capillary tubing (100 μm inner diameter, 365 μm outer diameter) using a laser puller (Sutter Instrument Co., model P-2000) and were packed with C18 reversed-phase material (Reprosil-Pur 120 C18AQ, 3 μm) in methanol using a pressure injection cell. .. Each pellet contained approximately 1 mg of biotinylated protein, as estimated using a peptide concentration assay of a streptavidin pulldown of the miniTurbo-3×FLAG-EGFP sample using a NanoDrop OneC (Thermo Fisher Scientific, Waltham, MA, USA) against a standard curve of a bovine serum albumin digest.

    Injection:

    Article Title: Optimizing Proximity Proteomics on the EvoSep-timsTOF LC-MS System.
    Article Snippet: All samples were acquired via data-dependent acquisition (DDA) LC–MS/MS using an Eksigent ekspert nanoLC 425 coupled to a TripleTOF 6600 Instrument (AB SCIEX, Concord, ON, Canada). .. Samples were resuspended in 5% formic acid, and aliquots equivalent to 1/8 and 1/4 of each sample (which were derived from one 150 mm plate, resulting in initial pellet masses of ∼50 mg) were directly loaded on a 100 μm × 15-cm nanospray emitter with a 5–8 μm tip opening at 800 nL/min for 20 min. Nanospray emitters were generated from fused silica capillary tubing (100 μm inner diameter, 365 μm outer diameter) using a laser puller (Sutter Instrument Co., model P-2000) and were packed with C18 reversed-phase material (Reprosil-Pur 120 C18AQ, 3 μm) in methanol using a pressure injection cell. .. Each pellet contained approximately 1 mg of biotinylated protein, as estimated using a peptide concentration assay of a streptavidin pulldown of the miniTurbo-3×FLAG-EGFP sample using a NanoDrop OneC (Thermo Fisher Scientific, Waltham, MA, USA) against a standard curve of a bovine serum albumin digest.



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry

    doi: 10.1016/j.xpro.2024.103286

    Figure Lengend Snippet:

    Article Snippet: Laser-based micropipette puller , Sutter Instrument , Cat#P-2000.

    Techniques: Recombinant, In Vitro, Transfection, Modification, Saline, Protease Inhibitor, Sequencing, Bradford Protein Assay, Software, Blocking Assay, Spectrophotometry, Extraction, Injection, Mass Spectrometry